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vko hela cells  (Addgene inc)


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    Addgene inc vko hela cells
    Vko Hela Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vko+hela+cells/GFP-mouse+vinculin+full+length+(889)+(Plasmid+%2367935)/pm37059721-250-8-17
    Average 91 stars, based on 4 article reviews
    vko hela cells - by Bioz Stars, 2026-10
    91/100 stars

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    Transfection:

    Article Title: In mitosis integrins reduce adhesion to extracellular matrix and strengthen adhesion to adjacent cells.
    Article Snippet: To re-express adhesome protein depleted HeLa cell lines, VKO HeLa cells were transiently transfected with pEGFP-mouseVinculin (#67935, Addgene) using lipofectamine 2000; TKO HeLa cells were retrovirally transfected with talin-head (pLCPCmod-hTalin1-headYpet) or full-length talin1 (pLPCXmod+hTalin–1(wt)-Ypet(short)) and KKO HeLa cells with kindlin2 (pRetroQ-mCherry-mKindlin2) using a standardized protocol61. .. To re-express adhesome protein depleted HeLa cell lines, VKO HeLa cells were transiently transfected with pEGFP-mouseVinculin (#67935, Addgene) using lipofectamine 2000; TKO HeLa cells were retrovirally transfected with talin-head (pLCPCmod-hTalin1-headYpet) or full-length talin1 (pLPCXmod+hTalin–1(wt)-Ypet(short)) and KKO HeLa cells with kindlin2 (pRetroQ-mCherry-mKindlin2) using a standardized protocol61. .. For virus production, respective transfer plasmids were transfected together with envelope plasmid pMD2.G (12259, Addgene) and packaging plasmid pUMVC (8449, Addgene) into HEK 293 T cells using lipofectamine 2000.



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    91
    Addgene inc vko hela cells
    Vko Hela Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/vko+hela+cells/GFP-mouse+vinculin+full+length+(889)+(Plasmid+%2367935)/pm37059721-250-8-17
    Average 91 stars, based on 1 article reviews
    vko hela cells - by Bioz Stars, 2026-10
    91/100 stars
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